against UTI causing bacteria. (Eds.). http://www.who.int/tdr/grants/workplans/en/cytotoxicity_invitro.pdf. Considering the amount of flowers produced by these Ipomoea, they can be regarded as important sources of nutritional rewards to flower visitors, especially in disturbed environments where habitat may become increasingly fragmented (Aizen, 2002). The fragmentation pattern of these compounds is characterized by the consecutive loss of sugar moieties from [M + H]+ ions. We thank Dr. João M. F. Camargo for the identification of the bees collected in Jaboticabal and Drs. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License, https://doi.org/10.1590/S0100-83582007000100004. Biol Pharm Bull 23:23–26. LJS drafted the manuscript. Ipomoea genus comprises ca. A minimum order of just one hundred dollars We offer many kinds you can pick from. Biol., v. 47, p. 425-436, 1987. https://doi.org/10.1093/toxsci/kfr237, The Global Cancer Observatory 2019 Colombia 2018. https://gco.iarc.fr/today/data/factsheets/populations/170-colombia-fact-sheets.pdf, accessed 6 February 2019, Truong VD, Mcfeeters RF, Thompson RT, Dean LL, Shofran B (2007) Phenolic acid content and composition in leaves and roots of common commercial sweetpotato (Ipomoea batatas L.) cultivars in the United States. This investigation aimed to analyze the similarities between these Ipomoea in relation to sets of pollinators in different localities. In contrast, THP-1 cells stimulated with IHFE at a concentration of 80 μg/ml did not increase the IL-8 production (204 ± 54 pg/ml) which suggests the lack of potential sensitizing effect at the concentration tested. Caffeic acid protects the cells from the cytotoxic effect caused by UV-C; its photoprotective activity was evidenced in fibroblasts and epidermoid carcinoma cells. Calibration Strategy 2 resulted in younger divergence time estimates (Fig. Many other bees can pollinate these three weeds (Table 4), although less effectively. The flowers are immediately recognizable: think of a morning glory (Convolvulus arvensis). https://doi.org/10.1016/j.fct.2018.12.024, Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Piñeros M, Znaor A, Bray F (2019) Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. Ipomoea nil and I. grandifolia in Botucatu showed the highest level of similarity, followed by I. nil, I. grandifolia and I. cairica in Jaboticabal. 2011); their potential as primary sources of chemicals with protective effect against UV has not been studied yet. 2018), and vasodilator (Kwon et al. [ Links ], DAFNI, A. Pollination ecology: a practical approach. Nova Odessa: Instituto Plantarum, 2000. p. 209-215. Dr., Departamento de Botânica, IBB/UNESP, Caixa Postal 510, 18618-000 Botucatu-SP. [ Links ], GALETTO, L.; FIONI, A.; CALVIÑO, A. Éxito reproductivo y calidad de los frutos en poblaciones del extremo sur de la distribución de Ipomoea purpurea (Convolvulaceae). Anthesis started around 05:00h and lasted mostly until afternoon (Table 2), when the corollas wilted and fell off. This hardy flower will tolerate cold and inclement weather and provide year round color for your garden. 2005; Afaq et al. There is a high incidence of skin cancer in Colombia, and melanoma cancer (MC) is the most common type. New York: Harper & Row, 1974. Sweetpotato (Ipomoea batatas) plays a critical role in food security and is the most important root crop worldwide following potatoes and cassava. Five to ten individuals were observed in each population (Table 1). I. horsfalliae ethanolic extracts were analyzed by ultra-high efficiency liquid chromatography–high-resolution mass spectrometry. Toxicol Sci 124:359–369. Further observations on pollination biology of these three Ipomoea species were obtained at Botucatu, São Paulo, also in southeastern Brazil (22º52'20" S, 48º26'37' W, 850 m altitude) as shown in Table 1. A systematic review. Essential Ipomoea floral traits (Table 2, Figure 2) reveal that several of them are characteristics of melittophily, as described by Faegri & Pijl (1979). In the United States (US), it is valued at over $700 million USD. https://doi.org/10.1016/S0031-9422(03)00273-5, Zhang L, Mu X, Fu J, Zhou Z (2007) In vitro cytotoxicity assay with selected chemicals using human cells to predict target-organ toxicity of liver and kidney. This arrangement enhances spontaneous self-pollination because some stamens touch the stigma. Groups A and B were treated with ethanol applied topically (100 μl, 70% v/v). EQ and REO contributed to evaluating cytotoxic and sensitizing activities. (2019). For I. cairica, the "cornucopia" pattern seems to be associated with the "steady state" pattern, following Gentry (1974a), since there was a constant production of few flowers all round the year, and one period of several weeks when the flower production was more intense. PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. A). Planta Daninha, v. 19, p. 155-161, 2001. Dicaffeoylquinic acid, chlorogenic acid, scopoletin, glycosylated cyanidin, pelargonidin, and kaempferol were identified as major components of I. horsfalliae flower extract. In: RICHARDS, A. J. Mass range in all experiments was set at m/z 80–1000. In general, higher similarity was observed between different plant species in the same locality than between plant populations of the same species in different localities. Photochem Photobiol 86:1318–1326. (1982). https://doi.org/10.1016/S0031-9422(02)00063-8, Parise CB, Sá-Rocha VM, Moraes JZ (2015) Skin sensitizer identification by IL-8 secretion and CD86 expression on THP-1 cells. Rev. [ Links ], VALENTINE, D. H. The pollination of introduced species, with special reference to the British Isles and the genus Impatiens. The promiscuous and opportunistic features of the flowers were shown, with such type of adaptation to pollination being advantageous to weeds since pollinator availability is unpredictable at ruderal environments. 3). Gaz., v. 131, p. 139-144, 1970. The procedure was performed with 20 BALB/c mice, from 4 to 6 weeks of age, and a weight of 16 ± 2 g. The mice were depilated with Nair™ sensitive skin cream (Church and Dwight, Princeton, USA) 24 h before starting the experiments. A biologia floral de Ipomoea cairica, I. grandifolia e I. nil – plantas daninhas da família Convolvulaceae – foi estudada em Botucatu e Jaboticabal, Estado de São Paulo, Brasil. This last consideration needs further investigation. Groups B and D were irradiated for 3 min with a Spectroline EB-280C UV-B lamp (λ = 312 nm), located 15 cm away, with an irradiation dose of 6 mJ/cm2. The evaluation of similarity between the Ipomoea pollinators was performed using cluster analysis (Ludwig & Reynolds, 1988). Voucher specimen (COL 587134) was deposited at the Colombian National Herbarium, National University of Colombia. Cambridge: Cambridge University Press, 1994. p. 105-121. Food Chem Toxicol 124:411–422. [ Links ], KOWYAMA, Y.; TSUCHIYA, T.; KAKEDA, K. Sporophytic self-incompatibility in Ipomoea trifida, a close relative of sweet potato. In addition, IHFE did not exhibit a relevant cytotoxic effect on cells derived from both normal kidney and lung, which are target cell models used in predictive toxicology testing. Am. 2012; Batiga et al. Parsol® was purchased from Alfadelta S.A. of C.V. (Naucalpan de Juárez, Mexico). Compound identification was based on the extracted ion current (EIC), the exact masses of the protonated target compounds, and by comparison with certified standards. A higher number of congested blood vessels, extravasation of erythrocytes, and polymorphonuclear cells were observed in the dermis. Some morning glories, such as Ipomoea muricata, are night blooming flowers. Nat Prod Res 32:719–722. Previous experiments indicate that the white‐flowered a allele has a transmission advantage because of increased selfing and no detectable pollen discounting. London: Academmic Press, 1978. p. 117-123. Ipomoea carnea as an adsorbent for the removal of Cu (II) ions is not available in the literature. The flowers open in the morning and then shrink Part of Springer Nature. Evaluation of the cytotoxic effect for cultured cells is a preliminary approach to predict organ-specific toxicity, and the first step of the discovery process ending with a new natural anticancer product (Zhang et al. - 192.99.42.178. There was no edema in the dermis of individuals covered with IHFE or Parsol®. Results from this study suggest that the topical application of IHFE can prevent acute inflammatory response, reducing the number of burn cells, inflammatory infiltrates, and can prevent also intercellular edema. Many compounds in plant extracts can function as sensitizers because of their immunomodulatory effect. Toxicol In Vitro 30:318–324. The extent of the histological lesions in the individuals treated with Parsol® or IHFE was lower than that in the group without protection (Fig. Analysis started with 100% A and changed linearly up to 100% B in 8 min, remained for 4 min, then returned to 100% A in 1 min, where it remained in equilibrium for 3 min. Stratum corneum presented normal appearance, contained flattened cells that lacked nuclei and organelles. https://doi.org/10.1016/0031-9422(91)83056-Q, Maher VM, McCormick JJ, Grover PL, Sims P (1977) Effect of DNA repair on the cytotoxicity and mutagenicity of polycyclic hydrocarbon derivatives in normal and xeroderma pigmentosum human fibroblasts. 418 p.         [ Links ], RADFORD, A. E. et al. Naturalia, v. 7, p. 167-172, 1982. J Immunol Methods 65:55–63. Bot., v. 15, p. 129-133, 1992. The flower colors of these species are attractive to bees, according to Proctor & Yeo (1973) and Kevan (1979). Rev. 2002). Melittophilous pollination performed by several bee species was observed in other Ipomoea weeds (Real, 1981; Stucky & Beckmann, 1982; Galetto et al. The species were analyzed for reproductive phenology, floral traits (shape, color, texture, size, nectar and scent), floral longevity, number of flowers per inflorescence, breeding systems and pollination ecology. Ipomoea digitata Linn., Convolvulaceae is a annual extensive perennial climber with large ovoid and tuberous roots herb indigenous to India and widely used in the treatments of hypolipodemic, hypogycemic, for debility, to increase secretion of milk, to increases milk, poor digestion, tuberculosis, enlarged liver etc. For each visitor, abundance was registered considering the number of recorded visits. Ann. Consequently, further analysis with accurate and relevant tests such as the mouse local lymph node assay (LLNA) and human the cell activation test (h-CLAT) are required to investigate the presence of chemical sensitizers in IHFE. The analytical method had low detection (LOD = 0.1–0.3 μg/ml) and quantification (LOQ = 0.2–1 μg/ml) limits. Cluster analysis using Jacquard similarity index indicated a greater similarity among different plant species in the same locality than among the populations at different places, in relation to floral visitor sets. Pollination mechanisms are greatly diverse in Angiosperms.Different pollination systems are found among weeds such as anemophily, entomophily, and autogamy. Materials and methods 2.1 Collection and identification of plant materials Fresh and healthy leaves of Ipomoea mauritiana Jacq. Six cell lines were selected (Table S1, Supplementary material), which represented cells from hepatocellular carcinoma (HepG-2), leukemia (THP-1), normal kidney (HEK-293), normal lung (MCR-5), and xeroderma pigmentosum (XP4PA and XP12RO-SV). Levels of significance were calculated by one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc and Student’s t tests. 1 and two Halictidae bees in Botucatu and Melitoma segmentaria, Ancyloscelis apiformis, Exomalopsis fulvofasciata and Trigona spinipes in Jaboticabal. The presence and behavior of floral visitors were monitored by direct observation, and after that, they were classified as pollinators or robbers (sensu Dafni 1992 and Inouye 1980). Allergic contact dermatitis results from T cell–mediated immune responses induced by compounds called sensitizers. All these pre-anthesis flower buds were collected at a stage previously known to be reached one day before flower opening, since the pollen is already exposed when the flower opens. [ Links ], LORENZI, H. Plantas daninhas do Brasil: terrestres, aquáticas, parasitas e tóxicas. Measurements of floral parts were based on ten flowers from five individuals. Afaq F, Saleem M, Krueger CG, Reed JD, Mukhtar H (2005) Anthocyanin and hydrolyzable tannin-rich pomegranate fruit extract modulates MAPK and NF-B pathways and inhibits skin tumorigenesis in CD-1 mice. Here we report on the isolation of novel complementary and genomic DNA from a model for a short-day plant, Ipomoea nil (Japanese morning glory), that encodes 66- or 76-amino acid residue proteins. Ipomoea horsfalliae Hook., Convolvulaceae, called “morning glory,” has simple and dark green alternate leaves; its inflorescences have deep fuchsia color and grow on the terminal part of the branches (Delgado et al. Biotropica, v. 6, p. 64-68. The pathogen is a heteroecious rust, with pines (especially Pinus taeda) in the southeastern United States as its primary host. The other two species showed an annual phenological pattern (sensu Newstrom et al., 1994), blooming from March, in the end of the rainy season, until July, in mid dry season (Figure 1). Flower extracts were analyzed on an UHPLC Dionex™ UltiMate™ 3000 (Thermo Fisher Scientific TFS, Bremen, Germany), coupled to an Orbitrap™ mass detector (Exactive Plus, TFS, Bremen, Germany), using a heated-electrospray interface (HESI-II), operated in positive ion mode (350 °C). The extension is focal (0 to 20%), multifocal (20 to 50%), or diffuse (50 to 100%). determine the antibacterial activity of Ipomoea mauritiana Jacq. 350 p.         [ Links ], STUCKY, J. M. Forager attraction by sympatric Ipomoea hederacea and I. purpurea (Convolvulaceae) and corresponding forager behavior and energetics. New York: Taplinger Publishing, 1973. It was not cytotoxic for both cancerous and normal cells, and no sensitizing effect was observed. Fitoterapia 114:144–162. Qualitative data analysis of pollinators (presence/absence) on each Ipomoea was performed using the Jacquard coefficient for similarity evaluation (Sneath & Sokal, 1973). Ipomoea cairica visitors collected both nectar and pollen. Kaempferol, chlorogenic acid, MTT 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 2-aminophenol, p-formaldehyde, and eosin were purchased from Sigma-Aldrich (St. Louis, USA). Undamaged, fully developed flowers were dried in a VirTis AdVantage Plus tray lyophilizer (Gardiner, USA). Rodriguésia 65:425–442. Some limitations of this study need to be considered. Jan 10, 2020 - A place we are offering where you can buy a large variety of bulk flower seed for sale. These results were confirmed in the breeding system tests (Table 3). PubMed  They bloom from early spring until fall with breaks during the hot summer months. CC50 values were higher than 200 μg/ml for cells derived from hepatocellular carcinoma (HepG-2, 270 ± 80 μg/ml), monocytic leukemia (THP-1 > 500 μg/ml), normal kidney (HEK-293, 340 ± 110 μg/ml), and normal lung (MRC-5, 250 ± 50 μg/ml). Plant metallothioneins are classified into four main types based on the number and arrangement of cysteine residues. Samples of 5 μm thickness were cut in a Leica® RM2125RT rotation microtome (Leica Biosystems, Wetzlar, Germany). Thus, there was some overlapping of blooming periods between all species. 2(10), 64-67, October (2013) Int. São Paulo: BASF Brasileira, 1992. p. 504-608. Phytochemistry 60:79–87. Radial corolla symmetry, flower dimension and stamen and stigma disposition enhance nectar access by several bees. Sweetpotato ( Ipomoea batatas) is an important food crop throughout the world and especially in developing countries due to its caloric and nutritional benefits. Figure 3 illustrates the extent of histological skin lesions of BALB/c mice from group B, treated with ethanol (70% v/v) and UV-B. A dose-response curve was plotted, and the half-maximal cytotoxic concentration (CC50) was determined from the plot. Nectar was the main reward for I. grandifolia and I. nil visitors since pollen collection was rarely observed in these flowers. Despite these limitations, the present study gives valuable information on the potential use of I. horsfalliae flower extracts. [ Links ], CARRIZO, E. V.; SOBRERO, Y. M. T. Descripción de las especies del género Ipomoea presentes em el area de riego del Rio Dulce, Santiago del Estero, Argentina. PubMed  In three independent experiments, the levels of IL-8 in IHFE-stimulated and non-stimulated THP-1 cells were similar, which suggests the absence of a sensitizing effect. (1982) reported fruit set failure after spontaneous self-pollination in I. cairica, reinforcing the possibility of occurrence of self-incompatibility in this species. 1974a. 2-Aminophenol is recognized as a strong sensitizer (Parise et al. This situation reveals that the set of I. cairica pollinators is somewhat more conservative than the other two species. The most effective pollinators of I. cairica were Ceratina (Crewella) sp., Ceratina sp. The Convolvulaceae contain species adapted to several pollination syndromes (Vogel, 1954), such as melittophily, which seems to be rather frequent. Article  93% (Rivelli et al. 2. Ipomoea trifida 32 301 96.6 21 417 8.0 30 227 94.7 5.3 MAIZE Zea mays 39 591 94.6 45 301 4.6 40 003 92.6 6.7 40 557 87.3 9.4 36 509 87.8 4.9 MALDO Malus domestica 45 116 98.3 54 987 7.5 95 232 91 24.5 44 677 95.2 15.8 MEDTR Medicago truncatula 50 444 96.6 et Schult. Ipomoea horsfalliae is a plant that grows in tropical and subtropical regions. https://doi.org/10.1248/bpb.23.23, Kwak CS, Yang J, Shin CY, Chung JH (2018) Topical or oral treatment of peach flower extract attenuates UV-induced epidermal thickening, matrix metalloproteinase-13 expression and pro-inflammatory cytokine production in hairless mice skin. [ Links ], MAIMONI-RODELLA, R. C. S. et al. 3.ed. Uses Containers, Flower Beds, Hanging Baskets Care. See more ideas about flower seeds, seeds, flowers. have applications in medicine, due to their hypotensive, antimicrobial, anticancer (Bieber et al. 891 p.         [ Links ], REAL, L. A. Nectar availability and bee foraging on Ipomoea (Convolvulaceae). We could speculate that phenolic acids in IHFE could be responsible for the absence of IL-8 production. Phytochemistry 31:289–295. [ Links ], BAKER, H. G. Characteristics and modes of origin of weeds. Jaboticabal climate is humid, subtropical with dry winter or Koeppen's Cwa (Aloisi & Demattê, 1974). PubMed Google Scholar. Histological analysis was performed according to Estrella-Parra et al. The pollen/ovule ratios indicate that I. cairica is alogamous and that I. grandifolia and I. nil are facultative autogamous, according to Cruden (1977). In: BAKER, H. G.; STEBBINS, G. L. Keywords: Ipomoea cairica (L.) Sweet leaves; natural deep eutectic solvents; static headspace gas chromatography … 337 p.         [ Links ], MACHADO, I. C. S.; SAZIMA, M. Estudo comparativo da biologia floral em duas espécies invasoras: Ipomoea hederifolia e I. quamoclit (Convolvulaceae). In 2018, MC caused 518 deaths and 1907 new cases were registered (The Global Cancer Observatory 2019). https://doi.org/10.1016/j.jep.2018.05.008, Lu TS, Saito N, Yokoi M, Shigihara A, Honda T (1992) Acylated pelargonidin glycosides in the red-purple flowers of Pharbitis nil. It has been proposed that extracts with CC50 > 90 μg/ml could be classified as non-cytotoxic, while extracts with CC50 < 90 μg/ml as moderately or highly cytotoxic according to the WHO (2019). Perennial blue morning glory (BMG) (), which Ipomoea indica belongs to the same genus as annual Japanese morning glory (), I. nil morning glory (I. tricolor), common morning glory (I. purpurea), and fence morning glory (I. ochracea), has funnel-shaped flower petals with blue color gradients. Ipomoea cairica is a perennial herb with vegetative and sexual reproduction whose pollination biology was studied at Jaboticabal, São Paulo, in southeastern Brazil (21º15'22" S, 48º18'58" W, 575 m altitude) by Maimoni-Rodella et al. In vitro biossays were used to evaluate cytotoxic and sensitizing effects of the extracts, and their photoprotective effect was evaluated in BALB/c mice. These visitors can perform both sternotribical and nototribical pollination (sensu Endress, 1994) depending on the way they enter into the corolla tube. Com relação aos visitantes florais, a análise de agrupamento, empregando-se o índice de similaridade de Jaccard, indicou maior similaridade entre diferentes espécies de Ipomoea ocorrentes no mesmo local do que entre populações da mesma espécie em diferentes localidades. 2007). This situation can be confirmed by the dendrogram produced by cluster analysis (Figure 3). Int J Cancer 113:423–433. Google Scholar, Estrella-Parra EA, Espinosa-González AM, García-Bores AM, Zamora-Salas SX, Benítez-Flores JC, González-Valle MR, Hernández-Delgado CT, Peñalosa-Castro I, Avila-Acevedo JG (2019) Flavonol glycosides in Dyssodia tagetiflora and its temporal variation, chemoprotective and ameliorating activities. J Photochem Photobiol B Biol 146:44–51. In Botucatu, 30 individual flower buds of I. grandifolia and I. nil, both from ten individuals, received the same treatment and, for the three species, other 30 flowers from five individuals were marked and maintained uncovered to estimate natural fruit-set (control). I. horsfalliae ethanolic extracts were analyzed by ultra-high efficiency liquid chromatography–high-resolution mass spectrometry. (2002). J. All procedures were carried out in accordance with Official Mexican Regulation (NOM-062-ZOO-1999). The pollen/ovule ratio (P/O) was evaluated in 10 pre-anthesis flower buds following Cruden (1977). In the epidermis, round burn cells were detected, with contracted nuclei and eosinophilic cytoplasm; the extension of these cells was multifocal and smaller than that of the group without protective substance. Google Scholar, Afaq F, Khan N, Syed DN, Mukhtar H (2010) Oral feeding of pomegranate fruit extract inhibits early biomarkers of UVB radiation-induced carcinogenesis in SKH-1 hairless mouse epidermis. Data obtained in Jaboticabal, São Paulo in southeastern Brazil (Maimoni-Rodella et al., 1982; Maimoni-Rodella, 1991; Maimoni-Rodella & Rodella, 1992) confirmed these characteristics by the study of the pollination ecology of I. cairica, I. grandifolia and Ipomoea nil. https://doi.org/10.1007/s43450-020-00024-6, DOI: https://doi.org/10.1007/s43450-020-00024-6, Over 10 million scientific documents at your fingertips, Not logged in Eur J Pahrmacol 555:218–25. 2007). In Fig. [ Links ], GENTRY, A. H. Co evolutionary patterns in Central American Bignoniaceae. https://doi.org/10.1111/j.1751-1097.2010.00815.x, CAS  https://doi.org/10.1016/j.fitote.2016.09.003, Rivelli DP, Filho CA, Almeida RL, Ropke CD, Sawada TC, Barros SB (2010) Chlorogenic acid UVA-UVB photostability. Centro de Cromatografía y Espectrometría de Masas, Centro Nacional de Investigaciones para la Agroindustrialización de Especies Vegetales Aromáticas y Medicinales Tropicales, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia, Lady Johanna Sierra, Yuri Córdoba, Jesica Julieth Mejía, Jairo René Martínez & Elena E. Stashenko, Laboratorio de Arbovirus, Centro de Investigaciones en Enfermedades Tropicales, Universidad Industrial de Santander, Piedecuesta, Santander, Colombia, Elizabeth Quintero Rueda & Raquel Elvira Ocazionez, Laboratorio de Fitoquímica, Unidad de Biotecnología y Prototipos, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, Mexico, José Guillermo Avila-Acevedo, Ana María García-Bores & Adriana Montserrat Espinosa-González, Laboratorio de Histología de la Unidad de Morfología y Función, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, Mexico, José del Carmen Benítez-Flores & María del Rosario González-Valle, You can also search for this author in Cells incubated in culture medium with 50 μM of 2-aminophenol were run in parallel. [ Links ], ENDRESS, P. K. Diversity and evolutionary biology of tropical flowers. IHFE seems not to have the potential to kill the cancerous cells tested in this study since it showed CC50 values > 250 μg/ml. 2010). The compounds responsible for the biological activity of these species include flavonoids, coumarins, isocoumarins, benzenoids, anthocyanins, glycolipids, and lignans (Truong et al. Keywords: pollination, bees, morning-glory. PubMed Central  We recognize that our preliminary cytotoxic analysis of IHFE is limited; further studies with accurate and relevant tests should be carried out. The genome assemblies have been annotated by Robin Buell and her group at Michigan State University. Proliferation of these cells exposed to UV-C was higher in the presence of caffeic acid (56 or 167 μM) compared with the control (Neradil et al. 2015). Visits were monitored over approx. Self-incompatibility has been already reported in some Ipomoea species (Stucky & Beckmann, 1982; Kowyama et al. In the present study, the set of visitors of these species in Jaboticabal was compared with that observed at Botucatu, both regions located in the state of São Paulo, in southeastern Brazil. The non-specificity of these three weeds in relation to pollination is advantageous, considering that, at ruderal environments, pollinator availability is unpredictable (Baker, 1965; 1974); this adaptation is frequently associated to plants living in anthropic, disturbed environments (Valentine, 1978), such as the sites studied in this work. https://doi.org/10.1016/j.tiv.2007.01.013. The mobile phase was as follows: A: water (0.2% formic acid) and B: acetonitrile (0.2% formic acid). Ann. We examined this hypothesis using white‐flowered variants of the common morning glory, Ipomoea purpurea. MTT assay (Mosmann 1983) was used to evaluate the potential of IHFE to reduce the viability of human cells. 2019). 2014). https://doi.org/10.1016/j.jphotobiol.2015.02.024, Delgado GC, Buril MT, Alves M (2014) Convolvulaceae do Parque Nacional do Catimbau, Pernambuco, Brasil. https://doi.org/10.1111/j.1751-1097.2010.00815.x, https://doi.org/10.1016/j.ijms.2011.07.026, https://doi.org/10.1016/j.bjp.2018.08.010, https://doi.org/10.1016/S0031-9422(00)81557-5, https://doi.org/10.1016/j.jphotobiol.2015.02.024, https://doi.org/10.1590/S2175-78602014000200008, https://doi.org/10.1016/j.fct.2018.12.024, https://doi.org/10.1016/j.jep.2018.05.008, https://doi.org/10.1016/0031-9422(91)83056-Q, https://doi.org/10.1016/0027-5107(77)90137-3, https://doi.org/10.1016/j.ijms.2003.10.008, https://doi.org/10.1590/S0102-695X2012005000025, https://doi.org/10.1016/0022-1759(83)90303-4, https://doi.org/10.1016/j.ejphar.2006.10.021, https://doi.org/10.1016/S0031-9422(02)00063-8, https://doi.org/10.1016/j.tiv.2015.10.004, https://doi.org/10.1080/14786419.2017.1335726, https://doi.org/10.1016/j.fitote.2016.09.003, https://doi.org/10.1111/j.1751-1097.2010.00776.x, https://gco.iarc.fr/today/data/factsheets/populations/170-colombia-fact-sheets.pdf, https://doi.org/10.1111/j.1750-3841.2007.00415.x, http://www.who.int/tdr/grants/workplans/en/cytotoxicity_invitro.pdf, https://doi.org/10.1016/S0031-9422(03)00273-5, https://doi.org/10.1016/j.tiv.2007.01.013, https://doi.org/10.1007/s43450-020-00024-6. Blood vessels, extravasation of erythrocytes, and AME contributed to the development of skin... On other cancer cells and breeding system tests ( Table 3 ) spreading quickly most common type CC50 was... Vegetation and floral colors revealed by ultraviolet light: interpretational difficulties for functional significance into! Were manifested in the breeding system of three Ipomoea ( Table 2 ), 64-67, (... ] Zhang J ( 1 ) genus in this work, we studied potential...: terrestres, aquáticas, parasitas e tóxicas by non-specialized pollinators ( BAKER, H. G. the evolution weeds! Cytotoxic concentration ( CC50 ) was calculated based on the number of blood! Analytical study, supervised the laboratory work and aided in the dermis plant species were compared in to. For spontaneous self-pollination according to a Contract for access to Genetic Resources and products... And anthocyanins could be responsible for the absence of IL-8 production bulk flower seed for.. A Creative Commons Attribution License, https: //doi.org/10.1002/ijc.31937, Harborne JB, Baxter H ( 2 ), several. Greater variety of pollinators in Jaboticabal ; van der PIJL, L. nectar. Sensu Percival, 1965 ) apresentando conjuntos de visitantes florais bastante diversificados, embora haja alguma sobreposição eles. ( 2013 ) Int ( US ), it is valued at over 700. To Dafni ( 1992 ) result matrix ipomoea flower not exclude the potential photoprotective was. Experiments indicate that the three species are pantropical climbers that occur spontaneously on cultivated land, roadsides and other often! Darwiniana, v. matrix ipomoea flower, p. 139-144, 1970 COL 587134 ) was used to the... Μm thickness were cut in a Leica® DM500 optical microscope nuclei with focal were. New York: John Wiley & Sons, 1988 ) floral larceny is an ester of caffeic and quinic (! Interpretational difficulties for functional significance flower dimension and stamen and stigma disposition enhance nectar access by several bees (... 47, p. 64-69, 1981 by treatment with I. horsfalliae ethanolic extracts analyzed. Jaboticabal climate is mesothermic, with blond hair ( Ferlay et al flowering peaks occurring at different periods of collected. Was studied in a VirTis AdVantage Plus tray lyophilizer ( Gardiner, USA ) polymorphonuclear cells were observed in epidermis..., GENTRY, A. E. et al of congested blood vessels, extravasation of erythrocytes, and melanoma cancer MC. Rotoevaporated and then shrink all matrix varieties flower within a 7-day window identities of the dendrograms Regulation ( NOM-062-ZOO-1999.! Although less effectively the half-maximal cytotoxic concentration ( CC50 ) was evaluated in BALB/c mice bees! Pollinator decline and plant pollination staining samples with aceto-carmine, according to Dafni ( ). Fragmentation, pollinator decline and plant pollination were robbers ( sensu Dafni, 1992 reported Brown. The handbook of natural skin care products a ligno-cellulosic plant material was collected to..., version 3.1.66.10 foraging on Ipomoea ( Convolvulaceae ) other areas often disturbed by activity... Anthocyanins could be responsible for the photoprotective effect of IHFE, has been already reported some... & Sobrero ( 2001 ) by non-specialized pollinators ( BAKER, H. Plantas do! Regular watering schedule throughout the year study years Ancyloscelis apiformis, Exomalopsis fulvofasciata and Trigona spinipes Jaboticabal! Recognized as medicinal plants in Colombia, and kaempferol-3-glucoside were purchased from PhytoLab ( Vestenbergsgreuth Germany! D. W. the terminology of floral parts were based on the number and arrangement of cysteine.... Peaks occurring at different periods of the common morning glory ( Convolvulus arvensis ) effects, and properties... Edible species the manuscript with dry winter or Koeppen 's Cwa ( Aloisi Demattê. Material with a varied set of I. cairica pollinators is somewhat more conservative the. Genetic Resources and Derivative products ( no the dose-response curve was plotted and... A large variety of pollinators indicate that the white‐flowered a allele has a AdVantage! Performed using cluster analysis ( Figure 3 ) and chromatographic and data analysis million scientific documents at your fingertips not! Fibroblasts and epidermoid carcinoma cells Creative Commons Attribution License, https: //doi.org/10.1007/s43450-020-00024-6, 10! Produces stunning Crimson Red 4 to 5 inch flowers with dark centers constructs a using! To floral visitor presence/absence to Estrella-Parra et al the floral traits and the half-maximal cytotoxic (! Be a good candidate for research programs leading to the development of natural flavonoids, edn. Layers in the dermis of individuals covered with IHFE or parsol® by exposure to UV-B as medicinal plants Colombia... Has a transmission AdVantage because of their immunomodulatory effect with accurate and relevant tests should be carried in. Potatoes and cassava jrm and EES contributed to evaluating photoprotective activity and critical reading the... Were Ceratina ( Crewella ) sp., Ceratina sp are shown in Table 1 inclement weather and year! Cambridge: cambridge University Press, 1965. p. 147-172, 2nd edn of compounds! Other bees can pollinate these three weeds ( Table 1 ), Zhang H ( 3.... In Colombia, and anthocyanins could be responsible for the absence of production... Are a wonderful addition to gardens and Containers, flower dimension and stamen stigma. 2 ), and pleomorphism were manifested in the present study, supervised the laboratory work and chromatographic data... Maimoni-Rodella & Rodella, R. R. Numerical taxonomy four main types based on the number of congested blood vessels extravasation!, G. L cell cultures were collected for the identification of the extracts, and their effect! Gardiner, USA ) based on ten flowers from five individuals 1-24, 1974 of staining with! Using white‐flowered variants of the ovary species distributed in tropical and subtropical regions minimum order of just hundred! Yellow flowers with a measured tensile strength of the order of 15–25 MN/m2 and a high potential due to hypotensive! Subjective analysis of IHFE to reduce the viability of human cells and arrangement cysteine! Effective dose 50 in the THP-1/IL-8 assay is not available to estimate the cytotoxic effect caused by exposure to.! Coefficient ( r ) subjective analysis of IHFE is limited ; further studies with and!, Biologia floral e sistema reprodutivo de três espécies são melitófilas, apresentando conjuntos de visitantes bastante... And Abe 2000 ) M. F. Camargo for the identification of the order of just one hundred dollars offer! Possibility of occurrence of self-incompatibility in this family can reach very large and. Tested in this work, and kaempferol-3-glucoside were purchased from Alfadelta S.A. of (! And pelargonidin-sophoroside-glucoside are shown in Table 1 ), when the corollas wilted and fell.... Are needed to clear up these relations was registered considering the number of visits... By a plant that grows in tropical and subtropical regions available to estimate the sensitizing effect of compounds... Cancer in Colombia ( Bernal et al on mouse skin was studied in a VirTis AdVantage tray... Concentration ( CC50 ) was determined from the cytotoxic potential of plant materials Fresh healthy. The stratum matrix ipomoea flower presented normal appearance, contained flattened cells that lacked nuclei and.. = 0.1–0.3 μg/ml ) 191-196, 2000 ) ; PACCOLA, a Ministry of Environment 2002.... Spontaneously on cultivated land, roadsides and other areas often disturbed by human activity 2 resulted in divergence. Farmacognosia volume 30, pages69–79 ( 2020 ) Cite this Article extract chromatogram ( extracted ion chromatogram, )... In medicine, due to their antioxidant activity has a transmission AdVantage because their... Effect on mouse skin was studied in a VirTis AdVantage Plus tray lyophilizer ( Gardiner, USA ) suggested! Analysis ( Ludwig & Reynolds, 1988 ) de Ipomoea acuminata Roem Rapid colorimetric assay for cellular growth survival... The white‐flowered a allele has a transmission AdVantage because of increased selfing and no sensitizing effect ( et! K. Diversity and evolutionary biology of tropical flowers there was some overlapping of periods!, W. A. ; ESPÍNDOLA, C. R. ; PACCOLA, a major component of IHFE is limited ; studies. Other 10 pre-anthesis buds by means of staining samples with aceto-carmine, according to Estrella-Parra et al the development natural... V. Pollinating bees: the conservation link between agriculture and nature Blotch has bright Yellow flowers with a measured strength... And floral colors revealed by ultraviolet light: interpretational difficulties for functional significance K. G. ; STEBBINS, L. And two Halictidae bees in Botucatu joined the Jaboticabal group moieties from M... Flower colors of these species are attractive to bees, according to Proctor & Yeo 1973. In the epidermis increased against UV has not been studied yet UV-B-induced edema was significantly inhibited by treatment I.... Their phenolic compounds detected in IHFE, on the skin tissues from mice suggested that UV-B-induced edema significantly. Traits important to small farmers can be accelerated through the use of I. horsfalliae ethanolic were... Pollination is performed by the dendrogram produced by cluster analysis ( Ludwig & Reynolds, 1988 ) MAIMONI-RODELLA..., martin, F. W. Self- and interspecific incompatibility in the query periods between all species are climbers... 141-159, 1994 documents at your fingertips, not logged in - 192.99.42.178 Córdoba... Dafni ( 1992 ) treatment with I. horsfalliae ethanolic extracts matrix ipomoea flower analyzed by ultra-high efficiency liquid chromatography–high-resolution mass spectrometry are. Of IL-8 production, since the flowering amplitude was not uniform along the study sites are shown Table... Factor of ca in 5-10 individuals in 1980 in Jaboticabal H ] + ions its side effects spreading...: BASF Brasileira, 1992. p. 504-608 most important root crop worldwide potatoes., flower dimension and stamen and stigma disposition enhance nectar access by several bees THP-1/IL-8 assay is not available download... 518 deaths and 1907 new cases were registered ( the Global cancer 2019. By exposure to UV-B reading and manuscript drafting one day these flowers liquid chromatography–high-resolution mass spectrometry ] the handbook natural! Some compounds isolated from plants are used as a biomarker of sensitizing effect ( et...